WebNow if you use SphI and SalI on the website I posted you can see that 2.1 or CutSmart buffer will give you 100% activity for SphI but 10% for SalI. You can use 3.1 that gives 100% for SalI and 50% ... WebBovine Serum Albumin (BSA) is supplied with some products to prevent adhesion of the enzyme to reaction tubes and pipette surfaces. BSA also stabilizes some proteins during …
BsaI-HF®v2 NEB
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BsaI NEB
WebApr 6, 2024 · NEB writes that BsaI digest does not require BSA for 100% activity at 50°C, but does for activity at the lower 37°C. Neither T4 nor T7 ligase buffers of Promega or … WebThermo Scientific Eco31I (BsaI) restriction enzyme recognizes GGTCTC (1/5)^ sites and cuts best at 37°C in G buffer (Isoschizomers: BsaI, Bso31I, BspTNI). See Reaction … WebSplit long sequences into smaller fragments that can be PCR amplified and are ready for assembly using Golden Gate. The NEBridge SplitSet™ tool available in the new NEBridge Ligase Fidelity Tool suite is a full-featured version of this utility that can provide results with higher fidelity and is recommended when the number of fragments is greater than 20. startheregoplaces.com