Cholesterol efflux assay kit cell-based

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August undefined, 2024

WebJan 8, 2024 · A cholesterol cell-based detection assay kit (10009779, Cayman Chemical) was used for histochemical identification of cholesterol. ... Cholesterol efflux was measured with a cholesterol efflux assay kit (ab196985, Abcam). BMDMs were incubated with Labeling Reagent + Equilibration Buffer mix containing ox-LDL (50 μg/mL) in the … WebApr 11, 2024 · A cholesterol uptake as well as a cholesterol efflux assay is described in detail, including suitable positive controls. We further show that Caco-2 cells can be efficiently transfected for luciferase reporter gene assays in order to determine nuclear receptor activation, main transcriptional regulators of cholesterol transporters (ABCA1, …

ab196985 Assay Kit (Cell-based) Cholesterol Efflux - Abcam

WebThe Amplex Red Cholesterol Assay Kit provides a sensitive and simple fluorometric method for detecting as little as 200 nM (or 80 ng/mL) of cholesterol in a 100 L volume. … WebSep 6, 2024 · This video demonstrates how to perform Cholesterol Efflux assay in cells using a simple, highly sensitive, add-incubate-read protocol. The results are quantitative and reproducible. Make... tem the boys na hbo

Cholesterol Uptake Capacity: A New Measure of HDL …

WebThe Cholesterol Efflux Assay Kit is a high-throughput screening assay that measures cholesterol efflux in cells using fluorescent-labeled cholesterol (λ Ex = 482 nm/ λ Em … WebCholesterol Efflux Assay Kit ab196985 is a high-throughput, cell-based screening assay for measuring cholesterol efflux. The cholesterol efflux assay protocol uses fluorescently-labeled cholesterol (Ex/Em 482/515 nm). It provides a safe, sensitive and reproducible … WebJan 15, 2024 · Cell-based Cholesterol Efflux Assay Kit (Fluorometric) is a high-throughput screening assay for measuring cholesterol efflux. storage instruction : Store the kit at … trenton on weater

Amplex™ Red Cholesterol Assay Kit - Thermo Fisher Scientific

Cholesterol Efflux Assay Kit - Sigma-Aldrich

WebThe Multidrug Resistance Direct Dye Efflux Assay Kit includes two of the best characterized and most commonly used multidrug resistance ABC transporter substrates, DiOC2 (3) and rhodamine 123. The kit enables researchers to directly assess the functional activity of the MDR1, MRP1 and BCRP membrane pumps in living cells under … WebJun 25, 2024 · Cholesterol efflux was determined using a cell-based Cholesterol Efflux Assay kit (Abcam, Cambridge, UK) according to manufacturer’s instructions. Activated cells were labeled with fluorescent cholesterol for 16 h and washed with the RPMI-HSA medium. A total of 5 μg protein weight of the treated HDL samples were added to the culture of ... tem the good doctor na netflixWebBioVision’s Cholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides … trenton orthopaedic group

"WebMay 18, 2024 · Macrophage foam cells (i.e., cholesteryl ester-laden macrophages) are abundant in atherosclerotic plaques, and increased macrophage foam cell content is associated with plaque instability (1, 2).Macrophages are generally thought to unload surplus cholesterol via efflux mediated by the ATP-binding cassette transporters A1 … " - Cholesterol efflux assay kit cell-based

Cholesterol efflux assay kit cell-based

Cholesterol Efflux Fluorometric Assay Kit (cell-based), …

WebThe Cholesterol Uptake Assay Kit is based on cellular uptake of a fluorescently tagged cholesterol probe. The fluorescence intensity measured at ex = 485 nm/ em = 535 nm is … WebCholesterol Efflux Fluorometric Assay Kit (Cell-Based) rev 3/19 (Catalog # K582-100; 100 assays; Store at -20°C) I. Introduction: Cholesterol efflux from the peripheral tissues …

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WebThe Cholesterol Efflux Assay Kit is a high-throughput screening assay that measures cholesterol efflux in cells using fluorescent-labeled cholesterol ( λEx = 485/ λEm = 523 nm). It is suitable for serum, isolated, or recombinant lipoprotein samples. It may also be used to screen small molecules for their effect on cholesterol efflux and ... WebBioVision’s Cholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides …

WebPrinciple of the Assay The Cell-based Cholesterol Efflux Assay Kit (Fluorometric) is a high-throughput screening assay for measuring ... cholesterol efflux from cells using a fluorescently-labeled cholesterol analogue. The kit includes a reagent to remove apolipoprotein B-containing lipoprotein particles (LDL and VLDL) from serum samples ... WebApr 10, 2024 · The intracellular cholesterol was measured according to the instructions of the intracellular total and free cholesterol assay kit for operation. Briefly, the cells were washed with PBS and lysed at room temperature for 20 min. The lysis fractions were collected and centrifuged, followed by protein and cholesterol quantification.

WebThe dysregulated homeostasis of cholesterol uptake, storage and efflux leads to the aggregation of foam cells under the arterial wall. At the same time, macrophages-secreted pro-inflammatory cytokines drive foam cells and vascular smooth muscle cells to migrate and proliferate, forming plaque lipid nuclei and fibrous caps [ 4–6 ]. Web产品概述. Cholesterol Efflux Assay Kit ab196985 is a high-throughput, cell-based screening assay for measuring cholesterol efflux. The cholesterol efflux assay …

WebShop BioVision Inc Cholesterol Efflux Fluorometric Assay Kit (cell-based) at Fishersci.com BioVision Inc Cholesterol Efflux Fluorometric Assay Kit (cell-based) …

WebJan 15, 2024 · Cell-based Cholesterol Efflux Assay Kit (Fluorometric) is a high-throughput screening assay for measuring cholesterol efflux. storage instruction : Store the kit at -20 C, protected from light. note : Result Data. type clonality : Kit. species reactivity cross reactivity : Mammals. application key : Func. size : 1 Kit. trenton order of battleWebCholesterol Efflux Fluorometric Assay Kit (cell-based) - 100 assays - K582-100 from BioVision. Tel: +44 (0)1223 316 855; register; log in; view basket: 0 items; ... BioVision’s … tem the big bang theory na amazonWebCholesterol Efflux Assay Kit (Cell-based) (ab196985) is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides a safe, sensitive and reproducible method for measuring cholesterol efflux. This product can be used to: ... tem themeWebCholesterol Efflux Fluorometric Assay Kit (Cell-Based) 1/14 (Catalog # MBS841611; 100 assays; Store at -20°C) I. Introduction: ... Thaw before adding to the cells. VIII. Cholesterol Efflux Assay Protocol: The procedure described below is for macrophage cell line J774.1. This procedure can also be used with macrophage cells derived from trenton oswal textile pvt ltdWebA negative correlation has been established between the in vitro efflux of cholesterol from macrophages and atherosclerosis. BioVision’s Cholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides a safe, sensitive, and reproducible method ... tem the last of us na netflixWebCell-based Cholesterol Efflux Assay Kit (Fluorometric) is a high-throughput screening assay for measuring cholesterol efflux. Supplier: Abnova™ KA4542 Catalog No. 89-939-181 tem thepWebApr 6, 2024 · For the oxidation-based quantification of cholesterol, total cholesterol was extracted using the cholesterol extraction kit (Sigma), and then oxidized using the Amplex Red Cholesterol Assay Kit ... tem thickness