WebOct 8, 2010 · IP with Orc2 antibody followed by immunoblot with ORCA polyclonal antibody (pAb) showed the association of Orc2 with endogenous ORCA (molecular weight of ORCA ∼70kDa; Figure 1 D). Finally, immunoprecipitation with ORCA pAb in human cells clearly demonstrated the association of ORCA with ORC subunits ( Figure 1 E). WebPreparation of the ORC2 antibodies. The Drosophila ORC2 coding sequence was PCR amplified from a 4- to 8-hr embryonic cDNA library (Brown and Kafatos 1988). The PCR product was subcloned into pCYB2 (New England Biolabs) usingNdeI and SmaI sites engineered into the termini of the PCR product. DmORC2 protein was expressed in …
Phosphorylation of ORC2 Protein Dissociates Origin …
WebJul 30, 2008 · We showed previously by coimmunoprecipitation that Drosophila Orc1 and Orc2 proteins interact with the dDP/dE2F1/Rbf1 complex in ovarian extracts, and we … WebApr 1, 1999 · We isolated mutations in Drosophila E2F and DP that affect chorion gene amplification and ORC2 localization in the follicle cells. In the follicle cells of the ovary, the ORC2 protein is localized throughout the follicle cell nuclei when they are undergoing polyploid genomic replication, and its levels appear constant in both S and G phases. dating a white rotary sewing machine
Phosphorylation of ORC2 Protein Dissociates Origin Recognition Compl…
WebThese antibodies target ORC2 in Human, Mouse and Rat samples. Our ORC2 polyclonal and monoclonal antibodies are developed in Rabbit and Rat. These antibodies have been … WebGene: orc-2 Immunogen: Recombinant His6-tagged ORC-2 fusion protein Alternate Gene Names: Alternate Antibody Name: 4D4 Clonality: Monoclonal Alternate Antigen Name: … WebAbout 20% of the Orc2 subunit is eluted from the nuclei after an RNase treatment ... anti-rabbit IgG (Amersham) and Alexa Fluor 488-conjugated goat anti-mouse IgG (Molecular Probes) were used as secondary antibodies. Drosophila cell culture extracts. Drosophila Schneider line 2 (S2) cells were maintained at 25°C in Schneider's Insect Medium ... bjshidaiqiche 126.com